Evaluation of Macroinvertebrate Field and Lab Sampling Methods Rigor CO_05_CMP12


1) Quantify the sampling precision of the single habitat (riffle) kick sampling protocol currently used by the Department and the precision of a multi-habitat sampling method being used by U.S. EPA and a number of other states. 2) Evaluate the sensitivity of single riffle and multi-habitat asssessment techniques for detecting 2 important stream stressors (eutrophication and sedimentation). 3) Document the sensitivity of current lab sub-sample size (125+ organisms), with 300+ and 500+ fixed counts to determine optimal sub-sample size. 4) Evaluate the sensitivity of Family-level, with Genus, and lowest practical taxonomic resolution (Genus-species) in detecting stream degradation.


Evaluate the accuracy, bias, sensitivity, and precision of various aspects of the Department's macroinvertebrate field and lab protocols to document methods' rigor and develop optimal (cost - data quality) methods.


1) Document within and between sampler variance for riffle and mutli-habitat sampling techiques. 2) Document the relative accuracy, bias,sensitivity, and precision of these two different sampling techniques in quantifying stream degradation that results form eutrophication and sedimentation. 3) Document the influences of laboratory sub-sample sizes at detecting stream degradation. 4) Document the influence of lab taxonomic resolution at detecting stream degradation. 5) Publish results in a peer-reviewed journal documenting methods' rigor. 6) Present findings to Monitoring Tech Team - and advocate changes in field or lab protocols if study finding suggest changes are warranted.

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Targeted Monitoring
Special Project
Reports and Documents
Documenting macroinvertebrate metric and index performance characteristics when using various field sample collection and lab sub-sampling protocols
Activities & Recommendations
Monitor Baseline Survey