NEW YORK BOTANICAL GARDEN: Desiccation,Freezing, and Dispersal of Nitellopsis obtusa

Purpose

NYBG will study germination & population genetics of a new invasive species to WI, starry stonewort (Nitellopsis obtusa). Experiments will assess effects of desiccation & freezing on SSW bulbils Genotypes and will be compared among SSW populations in WI, MN, MI, IN, NY and VT to determine priority locations for prevention efforts. Bulbils will be collected from Little Muskego Lake in WI & a lake in NY & used for lab experiments at NYBG to determine time necessary for freezing & desiccation treatments to reduce bulbil germination to zero. Bulbil germination success from several trials will be compared with bulbils grown under suitable lake conditions. The Dept will collaborate on the project by monitoring how SSW (& other aquatic plants) respond to a partial lake drawdown over the winter of 2016 in Little Muskego Lake. Pre & post point intercept plant surveys will take place in Hillview (Barefoot) Bay in 2016 & 2017. Soil cores will be collected at 20% of the P-I sites to estimate frost depth & soil moisture to determine if freezing and/or desiccation can control SSW bulbil germination. DNA extractions will be made from newly collected and existing collections of SSW at NYBG. Genotyping by sequencing (GBS) will then be carried out at the Genomic Diversity Facility at Cornell University. This info will be used to highlight the invasion pattern of SSW in North America. Biannual check-in meetings will be held & progress reports will be presented to the Dept Lakes Technical Team liaison. Results will be shared at meetings & published in peer-reviewed scientific journals. Scope Amendment Statement: NYBG will also organize and lead two trainings per year on the identification of starry stonewort and other Characeae for DNR staff, partners and citizens. The final report will be submitted in electronic format. It is recommended that the Grantee provide DNR liaison with a draft report prior to requesting final payment.

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Aquatic Invasives Grant
AIRD10716
2016
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Grant AIRD10716 awarded