Application Information
Apply for laboratory accreditation.
Program Information
More information about the LabCert program.
Resources & Training
Tools to assist you in testing.
Lab in Saukville

Lab in Saukville

Contact information
For information on the Lab Certification program, contact:
Rick Mealy
Program chemist
Science Services / Laboratory Certification
608-264-6006

DNR LabCert mailbox

Resources and assistance for Laboratories

Outreach is a critical component of the Laboratory Certification & Registration Program. We have compiled a list of forms, benchsheets, guidance documents, method references and training presentations to assist you in generating quality analytical results.

BOD resource

Welcome to the "Ultimate" BOD Resource. This web-based application is the culmination of eight years of training in both Wisconsin and Minnesota. To begin, simply click on a major subject button below or from the links at the side.

About BOD
A brief history of BOD including its origin and alternatives.
Sample handling
Sample handling information including holding time, preservation and storage information.
Sample pre-treatment
Information about the required preliminary testing associated with BOD.
Equipment & supplies
A look at the supplies and materials associated with BOD testing.
DO probe options
A look at DO probes and recent advancements.
Calibrating a DO meter
Perhaps the most important aspect of BOD testing: how to properly calibrate the DO meter and ensure acceptable blanks and QC.
Pressure adjustments & calibration
More detail regarding calibration. Specifically deals with how barometer pressure relates to calibration.
Method details
Information on specific aspects of BOD testing and method details including sample measurement and reporting.
BOD dilution water
Having good quality dilution water is critical for BOD testing. This section tells you how.
Seed & seeding issues
A detailed look at the importance of seed organism to BOD testing. Best sources of seed, how much to use and seed correction factor information.
Nitrifiers, nitrification and cBOD
A discussion of nitrification and the need to perform cBOD testing.
BOD sample toxicity/sliding BODs
How to determine if you have a toxic sample or if it looks like toxicity, but is something else.
BOD quality control
An overall summary of QA/QC requirements for BOD testing. Covers blanks, GGA, seed controls, replicates, control limits, and outlier testing.
Troubleshooting BOD results
Typical problems encountered in BOD testing and how to solve them.
Other BOD resources
A list of available books, websites and tools to guide you in your BOD testing.

Copyright 2006. University of Wisconsin Board of Regents.
Unauthorized use prohibited without the expressed written consent of the UW, State Laboratory of Hygiene and the DNR Laboratory Certification & Registration Program.

Forms

Many frequently requested documents are available for downloading.
Form description PDF format Alternate format
Federal Employer ID (FEIN)
Required for new or change -of-ownership applications. State Administrative Codes require that we obtain the FEIN of all individuals/businesses subject to any form of licensing.
Download file: 9400-568.pdf; Acrobat Reader; 48K
Application Form - Main
This is the main application form required for all application types.
Download file: Form 4800-002.pdf; Acrobat Reader; 120 K
Application attachment A1 (Aqueous)
Application attachment which indicates technologies and analytes being applied for in the AQUEOUS matrix.
Download file: App1AQr4.pdf; Acrobat Reader; 132 K
Application attachment A2 (Solid)
Application attachment which indicates technologies and analytes being applied for in the SOLID matrix.
Download file: App2SOr4.pdf; Acrobat Reader; 120 K
Application attachment A3 (Drinking water)
Application attachment which indicates "classes" and analyte-method combinations being applied for in the DRINKING WATER matrix.
Download file: App3DWr3.pdf; Acrobat Reader; 61 K
Application attachment B (Small WWTP labs)
Application attachment which indicates all of the technology - analyte combinations typically applied for by WWTP labs (NOTE: this is for the Aqueous matrix only).
Download file: AppWWTP.pdf; Acrobat Reader; 28 K
Chain-of-custody form
Sample chain-of-custody form that meets all the requirements of revisions to ch. NR 149.
Download file: COCform.pdf; Acrobat Reader; 16 K Download file: COCform.xls; 2003 MS Excel spreadsheet; 28 K
Corrective action log form
Sample corrective action log form that meets all the requirements of revisions to ch. NR 149.
Download file: CorrActionLog.pdf; Acrobat Reader; 16 K Download file: CorrActionLog.doc; 2003 MS Word document; 100 K
Equipment Maintenance Log Form
Sample equipment maintenance log form that meets all the requirements of revisions to ch. NR 149.
Download file: EquipMaintLog.pdf; Acrobat Reader; 16 K Download file: EquipMaintLog.doc; 2003 MS Word document; 52 K
GEMS Comma Delimited Electronic Submittal Format
Provides required GEMS data elements and comma-delimited format.
Download file: GW_E-DataFormat.pdf; Acrobat Reader; 864 K
LOD/LOQ Calculation and Validation Worksheet
Spreadsheet that can be used to determine LOD (MDL) according to EPA criteria (40 CFR Part 136 Appendix B).
Download file: LODCalcs.xls; 2003 MS Excel spreadsheet; 48 K
AutoPipet calibration verification worksheet
Spreadsheet that can be used to document quarterly verification of autopipets.
Download file: PipetCalcs.xls; 2003 MS Excel spreadsheet; 56 K
Newly received chemicals logbook
Spreadsheet that can be used to track chemicals upon receipt in the lab.
Download file: ReagentLog.xls; 2003 MS Excel spreadsheet; 24 K
Reagent/Standard preparation logbook
Spreadsheet which can be used to provide traceability for reagents or standards prepared in the lab using one or more chemical reagents.
Download file: StdPrepLog.xls; 2003 MS Excel spreadsheet; 24 K

Checklists

Method checklists

The focus of these checklists is to cover procedures or requirements that are specifically mandated by code or method. They do not cover good lab practices and may not cover every single requirement. It remains the lab's responsibility to ensure that all proper procedures are followed. However, labs may find that the checklists are a good self-auditing tool to make sure there are not any major requirements being overlooked. Your auditor will review these requirements at an evaluation, but they will also go beyond these lists to provide required changes and procedural recommendations that should improve your lab results.

Available checklists

Download a checklist from the following table.
Checklist Excel (.xls) PDF
BOD & cBOD checklist Download file: BOD-cBOD_chklst.xls; 2003 MS Excel; 32K Download file: BOD-cBOD_chklst.pdf; Acrobat Reader; 20K
TSS checklist Download file: TSS_chklst.xls; 2003 MS Excel; 24K Download file: TSS_chklst.pdf; Acrobat Reader; 12K
Ammonia checklist Download file: NH3_chklst.xls; 2003 MS Excel; 20K Download file: NH3_chklst.pdf; Acrobat Reader; 12K
Total phosphorus checklist Download file: TP_chklst.xls; 2003 MS Excel; 24K Download file: TP_chklst.pdf; Acrobat Reader; 16K
WWTP QC checklist Download file: QC_chklst.xls; 2003 MS Excel; 24K Download file: QC_chklst.pdf; Acrobat Reader; 16K
Full WWTP checklist Set Download file: WWTP4tests_chklst.xls; 2003 MS Excel; 80K Download file: WWTP4tests_chklst.pdf; Acrobat Reader; 48K

Benchsheets

These are benchsheets we have developed in order to assist your lab in compliance with NR 149 requirements. Benchsheets have been designed to address all critical method and code requirements for documentation and traceability.

Please note that benchsheets do change over time. Benchsheets are one of those things that can always be improved upon. So check this page frequently for updates. If the page has been updated in any way, the "Revised Date" at the bottom of the page will change.

Analytical benchsheets

Download a benchsheet from the following table.
Benchsheet Excel (.xls) PDF
BOD (and cBOD) benchsheet Download file: Bench_BOD.xls; 2003 MS Excel; 28K Download file: Bench_BOD.pdf; Acrobat Reader; 44K
TSS benchsheet - quarterly re-dry
Use this benchsheet if you dry samples overnight and perform a quarterly verification that drying time ensures drying to constant weight.
Download file: Bench_TSS_QTR.xls; 2003 MS Excel; 32K Download file: Bench_TSS_QTR.pdf; Acrobat Reader; 12K
TSS benchsheet - constant weight
Use this benchsheet if you dry samples for the minimum 1 hour and dry to constant weight.
Download file: Bench_TSS_CW.xls; 2003 MS Excel; 32K Download file: Bench_TSS_CW.pdf; Acrobat Reader; 40K
Ammonia (ISE) benchsheet Download file: Bench_NH3_ISE.xls; 2003 MS Excel; 48K Download file: Bench_NH3_ISE.pdf; Acrobat Reader; 44K
TP benchsheet - initial calibration
Use this benchsheet on days you are performing a full (initial) calibration.
Download file: Bench_TP_ICAL.xls; 2003 MS Excel; 52K Download file: Bench_TP_ICAL.pdf; Acrobat Reader; 44K
TP benchsheet - verify calibration daily
Use this benchsheet on days you are not performing a full (initial) calibration' i.e., on days after a valid calibration on which you simply VERIFY that the initial calibration remains valid.
Download file: Bench_TP_CCV.xls; 2003 MS Excel; 52K Download file: Bench_TP_CCV.pdf; Acrobat Reader; 44K

Other QC-related spreadsheets/forms

Download a benchsheet from the following table.
Benchsheet Excel (.xls) PDF
Pipet quarterly calibration worksheet Download file: PipetCalVerify_QTR.xls; 2003 MS Excel; 36K Download file: PipetCalVerify_QTR.pdf; Acrobat Reader; 12K

Methods

This page is split into three parts: (1) actual methods that are somewhat unique to the state of Wisconsin; (2) some supporting documents that are helpful; and (3) a list of excellent web resources to help you locate methods.

Download a method from the following table.
Method Format & link
Water extractable phosphorus (WEP)
Method for water extractable phosphorus (WEP) in biosolids. [March 2004] Pennsylvania State University Dept. of Agricultural and Biological Engineering
Download file: documents/methods/WEP_H2OExtPhos.pdf; Acrobat Reader; 36K
Wisconsin DRO method
Method for Wisconsin Diesel Range Organics (DRO) Procedure and Requirements. [September 1995]
Download file: documents/methods/DroSep95.pdf; Acrobat Reader; 76K
Wisconsin GRO method
Method for Wisconsin Gasoline Range Organics (GRO) Procedure and Requirements. [September 1995]. Also includes PVOC (Petroleum VOCs) requirements.
Download file: documents/methods/GroSep95.pdf; Acrobat Reader; 100K

Methods support information

Download method support information from the following table.
Document Format & link
BOD - Dilution Chart
Table to determine which dilutions are best for a particular sample based on expected BOD.
Download file: documents/methods/BOD_diliution_chart; Acrobat Reader; 16K
BOD - DO Saturation Chart
Table to determine which dilutions are best for a particular sample based on expected BOD.
Download file: documents/methods/DO_Sat_Table.pdf; Acrobat Reader; 40K
WSLH Ammonia Distillation Study
Copy of the study performed by the State Lab of Hygiene to demonstrate that distillation is not required for domestic wastewater effluents. If you analyze wastewater and do not distill your samples because of this study, then you are required to have a copy of this study on file.
Download file: documents/methods/NH3_DistStudy-SLH.pdf; Acrobat Reader; 40K
HACH Ammonia Distillation Study for Ammonia by Test n' Tube
Copy of the study performed by the HACH company to demonstrate that distillation is not required for domestic wastewater effluents. If you analyze wastewater and do not distill your samples because of this study, then you are required to have a copy of this study on file.
Download file: documents/methods/NH3-TNT_plus_report; Acrobat Reader; 40K
How to Apply for Phosphorus
Document produced by Laboratory Certification staff which outlines precisely what is required to apply to add phosphorus testing to your certification or registration. The concepts apply globally to any other testing you wish to add.
Download file: documents/methods/How2Apply4Phos.pdf; Acrobat Reader; 8K
PCB Screening in Drinking Water
The regulated parameter for drinking water is PCBs as decachlorobiphenyl. Labs are allowed, however, to screen for PCBs as Aroclors in certain circumstances. This document outlines requirements and criteria required to be certified to perform PCB screening of drinking water.
Download file: documents/methods/PCBscreenFlyer.pdf; Acrobat Reader; 112K
Interference Correction for ICP-OES
The simple reality is that approved methods for ICP-OES do a poor job of explaining how to address interference correction and, more importantly, how to properly and defensibly evaluate whether or not your correction routine is sufficient. This document highlights 20 critical things to know about interference correction and assessment.
Download file: documents/ICP-OES_Interference_correction.pdf; Acrobat Reader; 29K

Web methods resources

The links below will take you to the web sites maintained by the U.S. Environmental Protection Agency. The department does not endorse the ideas, organizations or products listed at these sites. These sites are presented as a tool to locate information on the Web.

National Environmental Methods Index (NEMI) [exit DNR]
EPA and USGS online database of methods and procedures for both regulatory and non-regulatory monitoring purposes for water, sediment, air and tissues.
Manual For The Certification Of Laboratories Analyzing Drinking Water [exit DNR]
Fifth Edition PDF (209pp, 4M) EPA 815-R-05-004, January 2005
Test Methods for Evaluating Solid Waste Physical/Chemical Methods [exit DNR],
SW-846, United States Environmental Protection, Office of Solid Waste, including updates and new methods.
EPA CWA Analytical Test Methods Home [exit DNR]
The Methods collection contains all approved and other unapproved test methods for Clean Water Act testing. Also includes alternative test procedure approval and validation procedures.
Recently Developed Methods from the EPA Office of Research and Development (ORD) [exit DNR]
EPA Safe Drinking Water Act methods 200.5, 321.8, 332.0, 415.3, 445, 521, 528, 529, 535, 549.2, and 556.
National Service Center for Environmental Publications (NSCEP) [exit DNR]
This site was designed to provide easy access to EPA's publications and simple navigation to other services which may aide you in locating environmental information.
EPA Manual of Manuals [exit DNR]
This document contains the titles, publication numbers, dates of publication, ordering information, abstracts, tables of contents, analyte-method cross reference lists, and Introductions to eight laboratory analytical chemistry methods manuals published between 1988 and 1995.
DNR Biomonitoring Manuals and information.
The State of Wisconsin Aquatic Life Toxicity Testing Methods Manual and other information on the Departments Biomonitoring Program.
Environmental Monitoring and Assessment Program Surface Waters: Field Operations Manual for Lakes [exit DNR]
EPA/620/R-97/001. U.S. Environmental Protection Agency, Washington D.C., June 1997.
Fish Sampling and Analysis  [exit DNR]
The purpose of this manual is to provide overall guidance to states on methods for sampling and analyzing contaminants in fish and shellfish tissue that will promote consistency in the data they use to determine the need for fish consumption advisories.
Evaluation of Dredged Material, Proposed for Discharge in Waters of the U.S.  [exit DNR]
Testing Manual, Inland Testing Manual, United States Environmental Protection Agency, Office of Water (4305) Department of the Army, US Army Corps of Engineers, EPA 823-B-98-004, February 1998.
EPA Whole Effluent Toxicity (WET Methods) [exit DNR]
Methods are guidelines establishing test procedures for data gathering and compliance monitoring under National Pollution Discharge Elimination System (NPDES) permits.
Lake Michigan Mass Balance Study Methods [exit DNR]
Describes the sampling and analytical methods used in the Lake Michigan Mass Balance Study Methods Compendium. The compendium is organized into three volumes that describe sampling procedures used in the study and analytical procedures used by the Principal Investigators.
Monitoring, Sampling, and Analysis, Office of Underground Storage Tanks, EPA [exit DNR]
A crucial part of cleanup actions is accurately and carefully measuring progress toward cleanup levels by monitoring sites, taking samples and analyzing these data to measure progress toward that goal.
Index to EPA Test Methods, EPA [exit DNR]
Index to EPA Test Methods, 2003 edition, is also available in Portable Document Format (PDF).
Links to Sources of EPA Test Methods on the Internet, EPA Region I [exit DNR]
Links to various EPA methods including air methods, lead in paint, monitoring well sampling, etc.
Analytical Methods Developed by the Office of Ground Water and Drinking Water [exit DNR]
EPA Safe Drinking Water Act methods 300.1, 314.0, 314.1, 317.0, 326.0, 327.0, 331.0, 515.3, 515.4, 526, 527, 531.2, 532, 552.3, 556, and 556.1.
Statement of Work, Contract Laboratory Program, Superfund, EPA [exit DNR]
CLP analytical services are defined in statements of work (SOWs). Statements of work provide the technical and contractual framework for commercial environmental testing laboratories to apply EPA CLP analytical methods for the preparation/isolation, detection, and quantitative measurement of organic target compounds and inorganic target analytes in water and soil/sediment environmental samples.
EPA's Field Analytic Technologies Encyclopedia (FATE) [exit DNR]
This online encyclopedia is intended to provide information about technologies that can be used in the field to characterize contaminated soil and ground water, monitor the progress of remedial efforts, and for confirmation sampling and analysis for site close out.

Guidance

Guide to NR149 revisions

NR 149 Summary Web Applet
The web page link above will pop open a new window that provides a cursory glance of major sections of the laboratory certification administrative rule (NR 149). Clicking on a link will pop up a smaller window which explains the rule in greater detail.

Quality manual template (4th Ed., June 2008)

The example Wastewater Treatment Plant Laboratory Quality Manual is designed to provide an example of the minimum documentation needed to fulfill the requirements of NR 149.37. This manual is not designed to be a complete guidance document for commercial laboratories, as it is written based on the analytical testing requirements associated with small wastewater treatment plants only.

Document/Description PDF format Alternate format
Quality manual template
This is an example quality manual and its intent is to demonstrate one way in which the requirements of NR 149.37 can be met. It is the responsibility of each facility to develop and follow their own unique quality manual.
Download file: A_QualMan_rev0.pdf; Acrobat Reader; 440K Download file: A_QualMan_rev0.doc; 2003 MS Word; 404K

General guidance documents

Download general program guidance documents
Document/Description PDF format Alternate format
Guidance on interference correction for ICP-OES
2014.
Download file: ICP-OES_Interference_correction.pdf; Acrobat Reader; 29 KB
ATP protocol & criteria (EPA)
Protocol for EPA Approval of New Methods for Organic and Inorganic Analytes in Wastewater and Drinking Water
Download file: -ATPcriteria.pdf; Acrobat Reader; 1.81 MB
Guidance on the use of discrete analyzers under EPA Clean Water programs
2005 EPA memo provides recommendations on the use of discrete analyzer instrumentation.
Download file: -DiscAnalyzr_Memo.pdf; Acrobat Reader; 600 KB
Tests for drinking water from private wells
Department of Natural Resources publication. This brochure gives information about several common contaminants found in private wells. It should help you decide when to sample your well and how often, how to find a certified laboratory and who to call for help.
Download file: -DWtests4PW.pdf; Acrobat Reader; 336 KB
Initial Demonstration of Capability (IDC) requirements
Suggestions for meeting the NR 149 requirements for initial demonstration of capability (IDC) when methods do not specify such requirements.
Download file: - IDCreqs.pdf; Acrobat Reader; 16 KB Download file: - IDCreqs.doc; 2003 MS Word document; 40 KB
Guidance on ignitability & reactivity testing of solids
Information from the DNR Waste Program that answer frequently asked questions regarding waste characteristic testing.
Download file: -Ignite_React.pdf; Acrobat Reader; 68 K
Analytical detection limit guidance document
This document interprets the Laboratory Certification Program's policy on LOD, and contains helpful hints and suggestions to assist laboratories calculate LODs and LOQs.
Download file: -LODguide.pdf; Acrobat Reader; 340 KB
Required LODs for BOD & TSS
Wisconsin Department of Natural Resources guidance for how to report LODs for BOD & TSS. Addresses concern that permittees inconsistently report BOD and TSS "less-than" results.
Download file: -LOD4BOD_TSS.pdf; Acrobat Reader; 40 KB
Procedure for dealing with outliers
Simple procedure for identifying outlier data.
Download file: -Outliers_Proc.pdf; Acrobat Reader; 16 K
Outliers - Grubbs test example
Step-by-step example of identifying outlier data using Grubbs test.
Download file: -Outliers_Grubbs.pdf; Acrobat Reader; 8 KB
PCB guidance for biosolids
Wisconsin Department of Natural Resources memo to permittees recommending a new analytical scheme for analysis of municipal and industrial biosolids which may contain low levels of PCBs. Contains detailed analytical and landspreading schema.
Download file: -PCB_BioSolids.pdf; Acrobat Reader; 52 KB
Pipette performance check SOP
Sample SOP from the WI State Lab of Hygiene for performing required quarterly verification of calibration for autopipets.
Download file: -PipetCal_SOP.pdf; Acrobat Reader; 36 KBK
Pipet verification calculations
One-page handout showing the calculations required to verify pipet accuracy.
Download file: -PipetCalcs.pdf; Acrobat Reader; 16 KB
Pipet verification - table of "z" factors
Table of "z" factors used in calculation of pipettor accuracy.
Download file: -Pipet_zTab.pdf; Acrobat Reader; 8 KB
QC Required for small WWTP
Handout summarizing required QC for the four basic wastewater parameters: BOD, TSS, ammonia and total phosphorus.
Download file: -QCreq_WWTP.pdf; Acrobat Reader; 108 KB
BioSolids and s. NR 151.07(2)
Guidance on how the application of wastes (biosolids, sludge and septage) impact the application of other nutrients, e.g., commercial fertilizer or manure, applied in accordance with a nutrient management plan developed under NR 151.07.
Download file: -SludgeNutMgmtPlan.pdf; Acrobat Reader; 28 KB
Acceptable weight determinations for TSS testing
TSS methods require weighing samples to "constant weight." To ensure program consistency, this letter clarifies available options constituting acceptable weight determinations for TSS.
Download file: -TSS_DryTime.pdf; Acrobat Reader; 36 KB
VOC monitoring in Wisconsin - which analytes are required?
To assist laboratories doing VOC testing, the Laboratory Certification Program put together a list of 68 commonly requested VOCs by all DNR programs. This is not an all-inclusive list.
Download file: -VOC_List_WI.pdf; Acrobat Reader; 24 KB

NR 149 implementation guidance

The following information can and should be used to help you develop procedures required by changes to NR 149 or to provide you with basic information regarding the structure and operation of the Laboratory Certification Program.

Information in this guidance will be updated over time. Please make sure to revisit this page and check to make sure you have the latest published version.

Download general program guidance documents
Document/Description PDF format Alternate format
Program administration Download file: 020ProgAdmin.pdf; Acrobat Reader; 65K Download file: 020ProgAdmin.doc; 2003 MS Word; 156K
Program "key dates" calendar Download file: 021KeyDates.pdf; Acrobat Reader; 65K Download file: 021KeyDates.doc; Acrobat Reader; 65K
Application process Download file: 03AppProcess.pdf; Acrobat Reader; 43K Download file: 03AppProcess.doc; 2003 MS Word; 198K
Proficiency testing (PT) Download file: 04PT.pdf; Acrobat Reader; 92K Download file: 04PT.doc; 2003 MS Word; 484K
Sample management Download file: 05SampleMgmt.pdf; Acrobat Reader; 72K Download file: 05SampleMgmt.doc; 2003 MS Word; 56K
Audit process Download file: 06AuditProc.pdf; Acrobat Reader; 28K Download file: 06AuditProc.doc; 2003 MS Word; 60K
Equipment maintenance
Support equipment Download file: 07Equip-Suppt.pdf; Acrobat Reader; 52K Download file: 07Equip-Suppt.doc; 2003 MS Word; 52K
Calibration Download file: 08Calibration; Acrobat Reader; 108K Download file: 08Calibration; 2003 MS Word; 120K
Quality control
LOD & LOQ Download file: 091LODLOQ.pdf; Acrobat Reader; 36K Download file: 091LODLOQ.doc; 2003 MS Word; 44K
QC sample types & sequence Download file: 092QC_SampSeq.pdf; Acrobat Reader; 32K Download file: 092QC_SampSeq.doc; 2003 MS Word; 60K
WWTP QC sequence Download file: 093WWTP_QCSeq.pdf; Acrobat Reader; 24K Download file: 093WWTP_QCSeq.doc; 2003 MS Word; 60K
Corrective Action Download file: 094CorrAction.pdf; Acrobat Reader; 40K Download file: 094CorrAction.doc; 2003 MS Word; 90K
Records & Traceability Download file: 11Traceability; Acrobat Reader; 52K Download file: 11Traceability; 2003 MS Word; 68K
SOP (Standard Operating Procedures) Download file: 12SOP; Acrobat Reader; 84K Download file: 12SOP; 2003 MS Word; 98K
Appendices
On-site evaluation SOP Download file: AuditSOP.pdf; Acrobat Reader; 23K Download file: AuditSOP.doc; 2003 MS Word; 60K
NR149 -Plain language version Download file: C_NR149-plang.pdf; Acrobat Reader; 23K Download file: C_NR149-plang.doc; 2003 MS Word; 60K

Other Guidance

Whole effluent toxicity (WET) program guidance document - Bureau of Watershed Management Biomonitoring Program

Tools for submitting groundwater monitoring data
Download ASCII file format and/or MS Excel spreadsheet to assist with electronic data submittals to DNR Waste Management Program (GEMS).

Submitting public drinking water monitoring data
Information on file format for electronic data submittal of public drinking water monitoring data to DNR.

Training

Training & workshop materials

Beginning in 1998, the Laboratory Certification program has been involved with developing and presenting training sessions on a wide array of subjects. You will find, below, links to a PDF copy of handouts from these sessions. The handouts match the PowerPoint slides from the sessions.

Either browse through all the tables below or use the links above to jump directly to a portion of the table that interests you.

Ammonia & Phosphorus
Document/Description Year Format
The Phosphorus Rule - Impact on Lab Testing
Discusses the 2010 Phosphorus rule, LOD needs, and capabilities of various analytical techniques to achieve new LODs. Reviews means to lower TP LODs.
2011 Download file: TP-rule.pdf; Acrobat Reader; 1.022 K
Ammonia & phosphorus update
Updated session on ammonia (ISE) and phosphorus (Colorimetric, manual).
2002 Download file: TP_NH32.pdf; Acrobat Reader; 864 K
Ammonia & Phosphorus original
Original session on ammonia (ISE) and phosphorus (Colorimetric, manual).
2001 Download file: TP_NH31.pdf; Acrobat Reader; 996 K
Phosphorus LOD study
Report on the ability to achieve required LODs under the new DNR phosphorus rules.
2011 Download file: SS1091.pdf; Acrobat Reader; 904 K
Balances
Document/Description Year Format
Balance Use & Verification
More in-depth review of critical considerations when using analytical or toploading balances.
2002 Download file: BalanceVer2002.pdf; Acrobat Reader; 2.92 MB
The videos below are actually part of the "Back to Basics" (2003) training for general lab practices, but seem better fits in this section.
Analytical Balance Stability: Doors Closed
This short video demonstrates analytical balance stability with both doors closed.
2003 Play/Download file: BalanceDC.wmv; Windows Media Video 188K
Analytical Balance Stability: 1 Door Open
This short video demonstrates how analytical balance stability is compromised when only a single "weighing door" is opened.
2003 Play/Download file: Balance1DO.wmv; Windows Media Video 784K
Analytical Balance Stability: 2 Doors Open
This short video demonstrates how analytical balance stability is further eroded when both "weighing doors" are opened.
2003 Play/Download file: Balance2DO.wmv; Windows Media Video 500K
Analytical Balance Stability: 2 Doors Open w/ movement nearby
This short video demonstrates the effect of small drafts--even from nearby movement-- on the reliability of an analytical balance when both "weighing doors" are open.
2003 Play/Download file: Balance2Dm.wmv; Windows Media Video 428K
Vibration Impact on a Toploader
Another short video demonstrating the effect of vibration on toploading balance stability.
2003 Play/Download file: BalanceVibe-TL.wmv; Windows Media Video 288K
Vibration Impact: Analytical Balance w/o Damping Pad
Short video demonstrating the effect of vibration on an analytical balance that is not stabilized with a damping pad. This balance is situated on a solid countertop only.
2003 Play/Download file: BalanceVibe-AB.wmv; Windows Media Video 184K
Vibration Impact: Analytical Balance w/Damping Pad
Short video demonstrating the effect of vibration reduction on an analytical balance that is is stabilized with a damping pad. This balance is situated on a marble damping pad.
2003 Play/Download file: BalanceVibe-AB-D.wmv; Windows Media Video 184K
Balance Vibration Impact Test
Short video demonstrating the conditions used to evaluate vibration effects.
2003 Play/Download file: BalanceVibeTest.wmv; Windows Media Video 184K
Balance Use & Verification
An overview of proper care, maintenance, and use of analytical balances.
2003 Download file: BalanceVer2001.pdf; Acrobat Reader; 2.92 MB
Back To Basics (General Chemistry, Lab Practices)
Document/Description Year Format
Basic Lab Practices
Covers a diverse array of topics including: Glassware Generalisms, Solution Solutions (Preparing Standards Perfectly), Proper Pipetting Principles and Balance Basics.
2006 Download file: Basics-LabPractices.pdf; Acrobat Reader; 4.60 MB
General Chemistry Relationships
Reviews the importance of General Chemistry Relationships (pH, alkalinity, and hardness) in water and waste treatment, particularly disinfection. Also provides a good background on arsenic and treatment options for private wells.
2002 Download file: Basics-GenChem.pdf; Acrobat Reader; 2.40 MB
Biochemical Oxygen Demand (BOD)
Document/Description Year Format
BOD Quality Control Issues, Toxicity, and Nitrification
This session covers GGA trouble-shooting as well as BOD toxicity. Most significantly, this session focused on nitrification and BOD and "what does nitrification look like?".
2010 Download file: BOD and nitrification.pdf; Acrobat Reader; 1,010K
BOD "201"
More detailed than BOD "101"; more in-depth review of critical BOD (Biochemical Oxygen Demand) issues.
2006 Download file: BOD201.pdf; Acrobat Reader; 2.92 MB
BOD "101"
An extensive look at the BOD (Biochemical Oxygen Demand) test, one of the most difficult tests to perform.
2002 Download file: BOD101.pdf; Acrobat Reader; 2.10 MB
BOD Data & Loading Fees
Full Title: Using BOD and COD Data to Assess Loading Fees. Presented real data showing how COD and BOD can be used to help municipalities determine chargeback fees, particularly when toxicity is involved.
2007 Download file: BOD_LoadFees.pdf; Acrobat Reader; 508K
Calibration Considerations
Document/Description Year Format
The Use of Higher Order Calibration Functions
This document provides an overview of concerns related to forcing a calibration through the origin, the use of curve weighting functions, and the use of non-linear algorithms.
2001 Download file: Calibration.pdf; Acrobat Reader; 92 K
Chemical Additions Monitoring for Water Supplies
Document/Description Year Format
Chemical Additions for Water Supply Systems
Interestingly enough, certification is NOT required to test for chlorine residual, phosphate, and fluoride as part of the chemical additions monitoring for municipal water. Concerns about that led to this training session which was repeated a number of times.
2001 Download file: Calibration.pdf; Acrobat Reader; 3.78 MB
Chlorine & Disinfection
Document/Description Year Format
Fundamentals of Chlorine Chemistry and Disinfection
Covers the fundamentals of chlorine chemistry as it relates to disinfection. Shows data to support that even low levels of chlorine are effective against bacteria and viruses.
2007 Download file: CL2Chemistry.pdf; Acrobat Reader; 812K
Simplifying Total Residual Chlorine Testing
Covers the analysis of total Chlorine residual (TCR) by both colorimetry (DPD) and ion selective electrode (ISE). Offers tips to get low LODs you need.
2006 Download file: CL2SimpleTRC.pdf; Acrobat Reader; 1.73 MB
Role of Chlorine in Dealing with Water Contamination
Covers chlorine efficacy, chloramine formation, getting to breakpoint, "shock" chlorinating private wells and water systems, hypochlorite vs. chlorine gas.
2005 Download file: CL2_WaterContam.pdf; Acrobat Reader; 1.14 MBK
Secrets of Breakpoint Chlorination (color)
Breakpoint chlorination made easy. Covers fundamentals of chlorine chemistry and how to test for and ensure you've reached breakpoint. (full color version)
2005 Download file: CL2Breakpoint-c.pdf; Acrobat Reader; 368K
Secrets of Breakpoint Chlorination (b&w)
Breakpoint chlorination made easy. Covers fundamentals of chlorine chemistry and how to test for and ensure you've reached breakpoint. (black&white version)
2005 Download file: CL2Breakpoint-bw.pdf; Acrobat Reader; 276K
Nuts & Bolts of Chlorine Chemistry
Done in concert with an E. coli testing session. Focus on why we need to chlorinate and dechlorinate, the effect of chlorine on E. coli, and provides in-depth review on how to test for chlorine residuals using the DPD and ISE methods.
2004 Download file: CL2Nuts_Bolts.pdf; Acrobat Reader; 4.32 MB
Getting the Best Chlorine Residual Data
Brief overview of critical considerations to ensure that the chlorine residual data you generate is of acceptable quality. Demonstrates ability to achieve TCR LOD of 0.037 ppm or less.
2003 Download file: CL2GetGoodTCRdata.pdf; Acrobat Reader; 3.18 MB
DPD Colorimetric Testing for Chlorine
Thorough step-by-step presentation on analysis of chlorine residual using the DPD (colorimetric method. Also reviews spectrophotometry and calibration statistics. Produced as a summer intern project.
2003 Download file: CL2DPDfull.pdf; Acrobat Reader; 2.04 MB
Residual Chlorine Using DPD (Windows Media Video)
Video (14:20) shows step by step preparation of calibration standards and analysis of calibration and standards using the DPD technique. "Vacu-vials" are used. Windows Media Player (.wmv) format.
2003 Play movie: CL2DPD-WMV.wmv; Windows Media Video 129 MB
Residual Chlorine Using DPD (QuickTime movie)
Video (14:20) shows step by step preparation of calibration standards and analysis of calibration and standards using the DPD technique. "Vacu-vials" are used. QuickTime (.mov) format.
2003 Play movie: CL2DPD-QT.mov; Quicktime Video 16.6 MB
Distance Learning
Document/Description Year Format
Technology for Creating Utility Training
Technology can be used to produce on-line (distance)training for water utility operators. Training can be designed to meet needs of water utilities and regulators.
2006 Download file: DistLearnOpp.pdf; Acrobat Reader; 928K
Hexavalent Chromium
Document/Description Year Format
Chromium (VI) Testing at the Wisconsin State Lab of Hygiene
Pat Gorski with the State Lab of Hygiene reviews the lab's protocol for obtaining quality hexavalent chromium data using solid phase extraction (SPE) followed by ICP/MS. This method can detect hexavalent chromium (Cr+6) down to 20 ppt (0.02 ppb).
2011 Link to streaming video;00:35:18
ICP & ICP/MS
Document/Description Year Format
ICP - You CAN'T Just Push Play: Part 1(Mediasite)
First of five parts of the streaming video generated during this session conducted March 25, 2014.
2014 Play Mediasite video ICP Part 1 of 5
ICP - You CAN'T Just Push Play: Part 2(Mediasite)
Second of five parts of the streaming video generated during this session conducted March 25, 2014.
2014 Play Mediasite video ICP Part 2 of 5
ICP - You CAN'T Just Push Play: Part 3(Mediasite)
Third of five parts of the streaming video generated during this session conducted March 25, 2014.
2014 Play Mediasite video ICP Part 3 of 5
ICP - You CAN'T Just Push Play: Part 4(Mediasite)
Fourth of five parts of the streaming video generated during this session conducted March 25, 2014.
2014 Play Mediasite video ICP Part 4 of 5
ICP - You CAN'T Just Push Play: Part 5(Mediasite)
Fifth and last of five parts of the streaming video generated during this session conducted March 25, 2014.
2014 Play Mediasite video ICP Part 5 of 5
ICP - You CAN'T Just Push Play (color)
This session was devoted entirely to interference correction. including IECs, FACT, and MSF. A whole new PT challenge sample was constructed, as well as a "Real World" sample designed to show the need for internal standard adjustment when faced with a "matrix". Screen scans explain the reason(s) why labs had trouble with specific elements. 5.3Mb. Download this for viewing on a PC screen.
2014 Download file: ICP2014-color.pdf; Acrobat Reader; 5.3 Mb width=
ICP - You CAN'T Just Push Play (black&white)
This session was devoted entirely to interference correction. including IECs, FACT, and MSF. A whole new PT challenge sample was constructed, as well as a "Real World" sample designed to show the need for internal standard adjustment when faced with a "matrix". Screen scans explain the reason(s) why labs had trouble with specific elements. 6.4Mb. Download this for printing to minimize use of toner.
2014 Download file: ICP2014-B&W.pdf; Acrobat Reader; 6.4Mb
ICP - Secrets for Determining IECs
A shorter presentation focusing on the art of proper determination of interelement correction (IEC) factors for successful ICP analysis.
2008 Download file: ICP-IEC Secrets.pdf; Acrobat Reader; 124 KB
Advanced ICP Tips & Techniques
This follow-up to the initial ICP training session focuses even more intently on interferences and background correction. The highlight of the session was the analysis, by participating labs, of a highly challenging PT sample designed by Wibby Environmental. Obtaining accurate results on "the Wibby" required a lab to be acutely aware of the myriad of interference problems associated with ICP technology.
2005 Download file: ICP2 11-15-05.pdf; Acrobat Reader; 1.81 MB
ICP from Ar to Zn
Excellent 2 day session covering basic aspects of ICP technology including theory, quality control, and a detailed section on interference identification and correction.
2003 Download file: ICP Training 2003.pdf; Acrobat Reader; 3.30 MB
Basic Principles of ICP/MS
Part of a 2-day ICP training session. The materials focus on providing an overview of ICP/MS technology including how the instrument operates.
2003 Download file: ICPMS basics.pdf; Acrobat Reader; 1.80 MB
Lab Math
Document/Description Year Format
Fear No Math!
Lab Math is not as difficult as believed. This session covers an array of topics such as So- Do U Sudoku?, Allergic to Algebra, C1V1≠R2D2, What did you Geomean by that?, Prozac for Linear Depression, The "Nerds" Equation, and Magical Math is not a Criss Angel Stunt.
2008 Download file: Math4Labs.pdf; Acrobat Reader; 0.99 MB
Mercury - Ultra Low
Document/Description Year Format
Low Level Mercury - Importance of Clean Sampling
Provides an overview of mercury contamination in the environment, closing with a detailed look at the "Clean Hands - Dirty Hands" technique for sample collection.
2005 Download file: Mercury-LL.pdf; Acrobat Reader; 4.80 MB
On-site Evaluation Process
Document/Description Year Format
The Audit Aftermath- Helping you respond to an on-site evaluation report
Once the coffee and donuts are gone and the audit chemist has left the building the ball id firmly in your court. This workshop looks at the right (and wrong) ways to respond quickly to resolve any issues identified in your evaluation report.
2013 Download file:Audit_Aftermath.pdf; Acrobat Reader; 928K
Operator Certification Exams
Document/Description Year Format
Preparing for the New Operator Certification Lab Exam
Provides a brief history of operator certification exam generation and changes being made to study guides. Also provides a discussion of how to properly prepare for exams using study guides by applying reading for comprehension and mnemonic skills.
2009 Download file: OpCertExamPrep.pdf; Acrobat Reader; 1.51 MB
Polychlorinated Biphenyls (PCBs)
Document/Description Year Format
PCB Analysis: Laboratory Certification Concerns
Presents the key considerations for PCB analysis. Looks at the rationale for calibrating with a "16/60" mix.
2003 Download file: PCBanalysis.pdf; Acrobat Reader; 192 KB
PCB Extract Cleanup: Why? How?
Focuses on the difficulties associated with analysis of PCBs in biosolids. Reviews types of cleanups and presents a recommended cleanup sequence best suited for biosolids.
2003 Download file: PCBcleanup.pdf; Acrobat Reader; 3.38 MB
PCB Cleanup Summary
A handful of slides that summarize the recommended cleanup protocol for PCBs in biosolids showing "before" and "after" screen captures of the GC/ECD chromatogram.
2003 Download file: PCBsummary.pdf; Acrobat Reader; 204 KB
Pipet Calibration & Maintenance
Document/Description Year Format
Pipet Calibration Video (Windows Media Video)
Video (5:08) shows step by step procedure for verification of auto pipet calibration. Windows Media Player (.wmv) format.
2007 Play movie: PipetVideo.wmv; Windows Media Video 33.5 MB
Calibration Verification of Mechanical Pipets
Presents the key considerations for verifying and documenting the calibration status of mechanical pipets.
2007 Download file: Pipet_calibration_ppt.pdf; Acrobat Reader; 1,005 KB
Mechanical Pipet Calibration SOP
Customizable SOP for the calibration verification of mechanical pipets.
2007 Download file: Pipet_calibration_SOP.pdf; Acrobat Reader; 32 KB
Mechanical Pipet Calibration Spreadsheet
Excel spreadsheet for documentation and calculation of mechanical pipet calibration.
2007 Download file: PipetCal_spreadsheet.xls; Microsoft Excel; 37 KB
Pipet Calibration Form Annual)
Form to document the annual calibration verification of mechanical pipets.
2007 Download file: Pipet_form_annual.pdf; Acrobat Reader; 12 KB
Pipet Calibration Form (Quarterly)
Form to document the quarterly calibration verification of mechanical pipets.
2007 Download file: Pipet_form_quarterly.pdf; Acrobat Reader; 12 KB
Pipet Calculations
Summary of calculations required for the calibration verification of mechanical pipets.
2007 Download file: Pipet_calculations.pdf; Acrobat Reader; 16 KB
Pipet Z-Factors
Table of Z-factors required for the calibration verification of mechanical pipets.
2007 Download file: Pipet_Z-Factors.pdf; Acrobat Reader; 8 KB
Quality Control/Quality Assurance
Document/Description Year Format
Calibration of Mechanical Pipettes in the Modern Wastewater Laboratory
Periodic calibration and preventive maintenance are, therefore, essential to ensure the integrity of laboratory results. This session shows step-by-step how auto-pipet calibration is verified, along with worksheets to use and documentation to retain.
2008 Download file: QC-AutoPipetCal.pdf; Acrobat Reader; 0.98 MB
Everything you wanted to know about a Lab Audit... but were afraid to ask!
This session reviews what can be expected during a lab audit. The audit can be broken down to: opening discussion, general housekeeping, sampling concerns, a detailed review of methods, records review, and QA/QC.
2007 Download file: QC-AuditRevealed.pdf; Acrobat Reader; 1.08 MB
Using QC Information to Assess Data Quality
This session discusses the limitations and merits inherent to each QC sample type and how to use of this information to better enable you to decide when and how sample results need to be qualified.
2005 Download file:QC2_AssessDQ.pdf; Acrobat Reader; 344K
Advanced QA/QC for Lab Testing
A very detailed look at QA/QC, notably containing a different way to look at calculating spike recoveries (which are frequently done incorrectly). ** Please note that some information changed with the 2008 changes to NR 149 **.
2003 Download file:QC-AdvancedQA.pdf; Acrobat Reader; 736 KB
QA/QC for Lab Testing
This initial review of basic QA/QC includes step-by-step instructions for doing linear regression and other QC calculations using inexpensive calculators. * Please note that some information changed with the 2008 changes to NR 149 **.
2000 Download file:QC-QATraining.pdf; Acrobat Reader; 688 KB
Lab Reagent Water Requirements
Document/Description Year Format
Reagent Water Concerns: in search of ultrapure water
A detailed review of reagent water criteria. Defines "ultrapure" water, reviews practicality of ASTM standard D-1193, explores the significance of conductivity and problems associated with it, outlines PRACTICAL reagent water monitoring tools and benchmarks.
2005 Download file: ReagentH2O.pdf; Acrobat Reader; 604 KB
Reporting Requirements
Document/Description Year Format
How To Read & Interpret a Lab Report - What You Don't Know CAN Hurt You!
Reviews NR 149 requirements for reports and how to tell if a lab is properly certified to perform testing. Are subcontracted results properly noted? ...a labs LODs/LOQ adequate? Is this the correct analyte "list"? Resources available to labs and data users.
2009 Download file: RPT-Interpret.pdf; Acrobat Reader; 1.17 MB
DMRs and Qualified Data
Reviews how to properly report QC exceedances on a DMR. What anlytes require that LOD and LOQ be reported? Discusses the concept that qualified data does not mean bad data.
2002 Download file: RPT-QualifyDMR.pdf; Acrobat Reader; 172 KB
How Do I Report THIS?!
Provides an overview of topics such as significant figures, how to report replicates, "<" and ">" results. Discusses specific data scenarios encountered with BOD and TSS.
2002 Download file: RPT-How2Report.pdf; Acrobat Reader; 172 KB
Last revised: Monday April 14 2014