CALIBRATION

• Calibration Trouble
• About Winkler...
• Apples-to-Apples?
• Water v. Air
• Air Calibration Error
• Doing it Right
• Calibration Procedure
• Calibration Summary
• DO Accuracy

BOD SUPPLIES

• Incubator
• Barometers
• BOD Bottles
• Bottle Cleaning
• Storing Dil. H2O
• In-line Filters
• Tubing Types
• Cleaning Tubing
• Pipet Types
• Thermometers
• DO Meter & Probe
• pH Meter & Paper
• Other Supplies

DILUTION WATER

• Preparation
• Mis-Conceptions
• Excess Depletion
• Troubleshooting
• Water Systems
• SLH Experience
• Simple Solutions
• Labware Cleaning
• Bleach Alternate

METHOD DETAILS

• Appropriate Dilutions
• Initial Dilutions
• Some Tips
• Measuring
• Measuring 2
• Pipet Types
• Need Extra Nutrients?
• Initial DO
• Incubation
• Calculations

NITRIFICATION

• Nitrogen Demand
• Nitrification?
• cBOD
• cBOD & Toxicity

OVERVIEW

• What is BOD?
• Why 5 Days?
• BOD Pyramid
• Not an easy test!
• Significance
• Test Limitations
• BOD Problems
• BOD Alternatives
• Why BOD?

CALIBRATION & PRESSURE

• Pressure Adjustment
• UN-Correcting Pressure
• DO Saturation Point
• UNcorrected Tables
• Corrected Tables
• Put in Perspective
• Pressure Changes
• Why Saturate Dil. Water?
• Other Altitudes
• Final Thoughts

SAMPLE PRE-TREATMENT

• Required Testing
• Sample pH
• Chlorine Residual
• Super-Saturation
• Documentation
• Checklist

DO PROBES

• Probe Advances
• Luminescence
• LDO Features
• LDO Approved?
• Maintenance
• On-board Tools (YSI)
• Gen. Maintenance
• Troubleshooting
•    Probe Calibration
•    Probe Malfunction

QA & QC

• Limit of Detection
• Blanks
• Seed Controls
• GGA
• Replicates
•     Range
•     RPD
•     Dependency
•     Range or RPD?
• Control Limits
•     Range
•     RPD
•     Calculations
• Outliers
• Corrective Action
• Qualifying Data
• Documentation

TROUBLESHOOTING

• Is it Contamination?
•     …Calibration?
•     …or Seed?
• Bugs? Dirty Dishes?
• Examples:
•     GGA ↑ Blank OK
•    GGA ↑ Blank ↑
• Dilution Water
• Disinfecting Tubing
• Cleaning Glassware
• Water Systems
• GGA Fails High
• GGA Fails Low
• Poor Precision

TOXICITY

• In a Nutshell
• Looks Like…
• Examples
• Reporting It

SEED & SEEDING

• Seed Source
• Preparing Seed
• PolySeed hydration
• BODSeed Hydration
• Adding Seed
• Seeding Bottles
• Is 2 mLs enough?
• Seed Correction
•   Seeded Bottles
•   Seeded Dil. Water
• Seed Controls
• Summary

SAMPLING & SAMPLE HANDLING

• Sampling
• SampleHandling

FREQUENTLY ASKED QUESTIONS

• General BOD questions
• Sample pre-treatment
• Method details questions
• QA/QC questions

RESOURCES

• Books
• Web Sites
• Other Tools